Design of Efficient and Safe Neural Stimulators by Marijn van Dongen & Wouter Serdijn

Author:Marijn van Dongen & Wouter Serdijn
Language: eng
Format: epub
Publisher: Springer International Publishing, Cham

The response to the stimulus is recorded by whole cell patch-clamping Purkinje cells in the voltage-clamp mode using electrodes (the same pipettes as the stimulus electrodes) filled with (in mM): 120 K-Gluconate, 9 KCl, 10 KOH, 3.48 MgCl2, 4 NaCl, 10 HEPES, 4 Na2ATP, 0.4 Na3GTP, and 17.5 sucrose, pH 7.25. The membrane voltage is kept at -65 mV and the membrane current is measured using an EPC 10 double patch clamp amplifier (HEKA electronics).

Two different kinds of stimulation are performed and the responses of the Purkinje cell are compared to each other. First of all classical stimulation is applied using a monophasic constant current source. For this purpose a Cygnus Technology SIU90 isolated current source is used. The amplitude of the current is varied to see the effect of stimulation intensity on the response of the Purkinje cell. The stimulation protocol consisted of two consecutive stimulation pulses with a duration of each and an interpulse interval of 25 ms.

Second, switched-mode stimulation is performed, also using two pulses with and an interpulse interval of 25 ms. If the Purkinje cell shows a similar response for varying δ during switched-mode as it does for varying amplitude during classical stimulation, it can be concluded that switched-mode stimulation is indeed able to mimic classical stimulation.

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